Background: WWP2 is a HECT domain containing E3 ubiqutin protein ligase. WWP2 specifically interacts with Rpb1 and is responsible for targeting it for ubiquitination. Atrophin interacting proteins (AIP)s bind to atrophin-1 in the vicinity of the polyglutamine tract. The WW domain consists of 35-40 amino acids and is characterized by four well conserved aromatic residues, two of which are tryptophan. All five AIPs contain multiple WW domains and can be divided into two distinct classes. AIP1 and AIP3 (WWP3) are MAGUK-like multidomain proteins containing a guanylate kinase-like region, two WW domains and multiple PDZ domains. AIP2 (WWP2), AIP4 (itchy) and AIP5 (WWP1) are highly homologous, each having four WW domains and a HECT domain characteristic of ubiquitin ligases. These interactors are similar to isolated Huntingtin-interacting proteins, suggesting commonality of function between two families of proteins responsible for similar diseases.
Description: Rabbit polyclonal to WWP2
Immunogen: KLH conjugated synthetic peptide derived from WWP2
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 99 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
