Background: The Cdc2 p34-cyclin B complex plays a critical role in the cell cycle by regulating the G2 to M phase transition. Also referred to as M phase promoting factor or MPF, this complex is a required component of the cell cycle machinery and is necessary for cell entry into mitosis. In Saccharomyces cerevisiae, this complex is known as Cdc28 and is associated with two proteins whose human homologs are called Cks1 and Cks2. Cks2 (Cyclin-dependent kinases regulatory subunit 2) is a 79 amino acid protein that binds to the catalytic subunit of cyclin-dependent kinases, such as those in the Cdc2 p34-cyclin B complex. An essential component of this cyclin/cyclin-dependent kinase complex, Cks2 contributes to cell cycle control and is able to form a homohexamer that can bind up to 6 subunits. Without proper activity of Cks2, the first metaphase/anaphase transition of meiosis can not occur.
Description: Rabbit polyclonal to CKS2
Immunogen: KLH conjugated synthetic peptide derived from CKS2
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 10 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
