Background: The nucleolus consists of a number of specific proteins that play a critical role in the assembly of ribosomes, as well as in the maintenance and structural integrity of the nucleolus. NSUN1 (NOL1/NOP2/Sun domain family, member 1), also known as NOL1, p120, NOP120, NOP2 or Nucleolar protein 1, is an 812 amino acid nucleolar protein belonging to the methyltransferase superfamily. It is expressed in the G1 phase of the cell cycle and peaks during the early S phase. Considered a ribosomal RNA methyltransferase, NSUN1 may be involved in regulating the cell cycle and in increasing nucleolar activity that is associated with cell proliferation. NSUN1 is a possible marker for proliferation in neoplastic cells and in several cancer cells. Two isoforms exist due to alternative splicing events.
Description: Rabbit polyclonal to NOL1
Immunogen: KLH conjugated synthetic peptide derived from NOL1
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 95 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
