Background: Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of poly-peptides that contribute to mRNA transcription and pre-mRNA processing, as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which is the transcript produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein components are localized to the nucleus; however, some shuttle between the nucleus and the cytoplasm. Two abundant and ubiquitously expressed members of the hnRNP family are hnRNP D0 and hnRNP R. Specifically, the hnRNP D0 protein contains two RNA-binding domains (RBDs), which bind to both RNA and DNA sequences. hnRNP D0 also possesses a transactivator domain and is involved in transcriptional regulation.
Description: Rabbit polyclonal to hnRNP R
Immunogen: KLH conjugated synthetic peptide derived from hnRNP R
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 71 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
