Background: Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Subunit is monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric APAF1 and pro-caspase-9 bind to each other via their respective N-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the APAF1-pro-caspase-9 complex via interaction with pro-caspase-9. [tissue specificity] Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Description: Rabbit polyclonal to APAF1
Immunogen: KLH conjugated synthetic peptide derived from APAF1
Specificity: ·Reacts with Human, Mouse, Cow, Dog and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/200-500. Predicted Mol wt: 141 kDa;
·Immunohistochemistry (Frozen/paraffin tissue section): 1/100-500;
·Immunocytochemistry: 1/100-500;
·ELISA: 1/1000;
·Optimal working dilutions must be determined by the end user.
