Background: sPECAM-1 (soluble platelet/endothelial cell adhesion molecule-1) is referred to as CD31d. PECAM-1 (platelet endothelial cell adhesion molecule-1) also called CD31 and EndoCAM is a newly characterized adhesion molecule that belongs to the immunoglobulin superfamily. PECAM-1 is a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation. PECAM-1 is constitutively expressed on all vascular cells and has provided a useful immunohistochemical marker of blood vessels, particularly in the setting of angiogenesis. Besides the membrane-bound form of PECAM-1, a soluble form of the molecule exists, which is 5-10 kDa smaller than cell-associated PECAM-1, and contains the cytoplasmic tail. This form of soluble PECAM-1 (sPECAM-1/CD31d) is encoded by an alternatively spliced mRNA from which the exon containing the transmembrane domain has been removed. CD31d was detected in normal human plasma.
Description: Rabbit polyclonal to CD31d
Immunogen: KLH conjugated synthetic peptide derived from CD31d
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 125 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/100-500;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
