Background: Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. Epoxide hydrolase (EHs) are biotransformation enzymes that catalyze the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. The enzymatic hydration is essentially irreversible and produces mainly metabolites of lower reactivity that can be conjugated and excreted, and, therefore, are generally regarded as detoxifying. Soluble EH (sEH) is a ubiquitous mammalian enzyme for which liver and kidney are reported to have the highest activity. Microsomal EH (mEH) exhibits a broad substrate specificity, while sEH is an enzyme with a "complementary" substrate specificity to mEH. sEH is expressed in 3T3 and HeLa cells. sEH is encoded by the EPHX2 gene, which maps to chromosome 8p21.2.
Description: Rabbit polyclonal to EPHX1
Immunogen: KLH conjugated synthetic peptide derived from EPHX1
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 53 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/100-500;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
