Background: Microsomal glutathione transferase 1 (MGST1) is an abundant protein that catalyzes the conjugation of electrophilic compounds with glutathione, as well as the reduction of lipid hydroperoxides. The level of MGST1 mRNA in rat are highest in liver, while the levels of expression vary in non-hepatic tissues. In addition, the subcellular localization patterns of MGST1 are highly tissue specific. In hepatocytes and bile ducts, MGST1 is localized to the cytoplasm. MGST1 is localized to the nucleus in choroid plexus and primary spermatocytes and displays a granular cytoplasmic staining pattern in the adrenal medulla. Also, focal staining of MGST1 is observed in the distal tubules and collecting ducts of the liver. The human MGST1 gene maps to chromosome12p12.3-p12.1. Sequence comparisons of MGST1 with related proteins, including PIG12, FLAP, LTC4 synthase, MGST2 and MGST3, suggest that they are members of a dispersed microsomal GST gene superfamily. MGST1 is strongly inhibitied by leukotriene C4 and weakly inhibited by other leukotrienes, indicating a role for MGST1 in the cellular processing of leukotrienes.
Description: Rabbit polyclonal to MGST1
Immunogen: KLH conjugated synthetic peptide derived from MGST1
Specificity: ·Reacts with Human, Mouse, Pig and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 18 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
