Background: Glomerular visceral epithelial cells, also known as podocytes, maintain the selective filtration barrier of the renal glomerulus. NEPH1, a member of the immunoglobulin superfamily, plays a critical role in functional barrier development. Loss of NEPH1 expression, like that of its structural relative nephrin, results in nephrotic syndromes and proteinuria leading to perinatal death. NEPH1 associates with nephrin as well as ZO-1 and localizes with them to the glomerular slit diaphragm. Interaction with nephrin occurs via the extracellular domain of NEPH1 and with ZO-1 in a PDZ binding motif of the cytoplasmic tail. Mutation of a putative threonine phosphorylation site within the cytoplasmic domain abrogates interaction with ZO-1, implying that phosphorylation regulates this interaction, and may effect the recruitment of the appropriate signal transduction components to the complex.
Description: Rabbit polyclonal to NEPH1
Immunogen: KLH conjugated synthetic peptide derived from NEPH1
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 66 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/100-500;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
