Background: Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of 2 heavy chains (MHC) and 4 light chains (MLC) that interacts with actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. MYH14 (myosin, heavy chain 14), also known as myosin-14, non-muscle myosin heavy chain IIcDFNA4, MHC16, myosin, FLJ13881, FLJ43092, FP17425, KIAA2034, NMHC-II-C or DKFZp667A1311, is a 1,995 amino acid protein belonging to the non muscle myosin II family. MYH14 contains an N-terminal myosin domain, a C-terminal myosin tail, a myosin head region and two IQ domains. Four MYH14 isoforms exist as a result of alternative splicing and MYH14 is highly expressed in skeletal muscle, small intestine and colon. A form of sensorineural hearing loss known as non-syndromic sensorineural deafness autosomal dominant type 4 (DFNA4), is caused by MYH14 defects.
Description: Rabbit polyclonal to MYH14
Immunogen: KLH conjugated synthetic peptide derived from MYH14
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 228 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
