Background: The T-box transcription factor T-bet is considered to be a master regulator of Th1 lymphoid development controlling the production of the cytokine IFN gamma. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL12 stimulation and can be suppressed by addition of the Th2 cytokine IL4 or neutralization of IL12.
Description: Rabbit polyclonal to T-bet
Immunogen: KLH conjugated synthetic peptide derived from T-bet
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 58 kDa;
·Immunohistochemistry (Frozen/paraffin tissue section): 1/50-200;
·Immunocytochemistry: 1/100;
·ELISA: 1/500;
· Optimal working dilutions must be determined by the end user.
