Background: Myosin is a 480 kD protein that interacts with actin in muscle and non muscle cells. Conventional myosin contains two identical heavy chains (MHC-200kD each) and four light chains (MLC-15-26 kD). Conventional myosin molecules consist of two major regions: tail (rod) and globular heads; they aggregate into filaments through the tail region and interact with actin and with adenosine triphosphate (ATP) through the head region. Multiple forms of myosin heavy chains exist in skeletal, smooth and cardiac muscle and in non-muscle tissues. A spectrum of skeletal muscle fiber types is found in adult skeletal muscles. The two major skeletal muscle fiber types are type I (slow-twitch) and type II (fast-twitch). Skeletal muscle type II fibers can be further subdivided into types IIa (fast red) and IIb (fast white).
Description: Rabbit polyclonal to Fast skeletal Myosin
Immunogen: KLH conjugated synthetic peptide derived from Fast skeletal Myosin
Specificity: ·Reacts with Human, Mouse, Rabbit, Pig, Dog and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 19 kDa;
·Immunohistochemistry (Frozen/paraffin tissue section): 1/100-500;
·Immunocytochemistry: 1/100-500;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
