Background: T cell receptors activate immune responses by recognizing antigen and initiating a cascade of intracellular signal transduction events, eventually culminating in cell proliferation and differentiation. Both protein tyrosine kinases and PLCγ are activated by this event. LAT, or linker for activation of T cells, is an integral membrane protein that has been shown to associate with PLCγ1, as well as GRB2 and the p85 subunit of PI 3-kinase. Binding of these signaling molecules to LAT is associated with phosphorylation of LAT by ZAP-70/Syk tyrosine kinases. LAT appears to play a role in activation of transcription mediated by AP-1 and NF-AT following stimulation of the T cell receptor, suggesting that it acts as a linker protein in T cell activation. LAT protein is palmitoylated, and this modification is required for its tyrosine phosphorylation and localization to glycolipid-enriched microdomains.
Description: Rabbit polyclonal to LAT
Immunogen: KLH conjugated synthetic peptide derived from LAT
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 38 kDa;
·Immunohistochemistry (Frozen/paraffin tissue section): 1/50-200;
·Immunocytochemistry: 1/100;
· ELISA:1/500;
·Optimal working dilutions must be determined by the end user.
