Background: The Pax family encodes transcription factors that function during embryogenesis and regulate the temporal and position-dependent differentiation of cells. PAX8 is expressed in the developing and adult thyroid, the developing secretory system and at lower levels, in the adult kidney. PAX8 complexes with TTF-1 and TTF-2 to induce thyroid follicular cell differentiation and thyroid hormone biosynthesis by regulating the expression of sodium iodide symporter (NIS), thyroid peroxidase (TPO), thyroglobulin (TG) and the thyrotropin receptor (TSHR). Treatment of FRTL-5 cells with TGFβ1 decreases PAX8 mRNA levels and PAX8 DNA binding activity, which suppresses the expression of TG and the formation of thyrocytes. Patients who have autosomal dominant mutations of the PAX8 gene develop thyroid dysgenesis. The PAX8 gene produces six isoforms, A to F, that are generated by alternative splicing and differ in their carboxy-terminal regions. The PAX8 isoforms display different DNA binding capacities and are thought to be functionally distinct.
Description: Rabbit polyclonal to PAX8
Immunogen: KLH conjugated synthetic peptide derived from PAX8
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 48 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
