Background: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Description: Mouse monoclonal to FAK
Immunogen: Purified recombinant fragment of human FAK expressed in E. Coli.
Specificity: ·Reacts with Human and Mouse.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 125 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/1000;
·Optimal working dilutions must be determined by the end user.
