Background: Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that can be activated by a variety of compounds including fibratus, thiazolidinediones, prostaglandins and fatty acids. Three PPAR subtypes, designated PPARα, PPARβ (also designated PPARδ) and PPARγ, have been described. PPARs promote transcription by forming heterodimers with members of the retinoid X receptor (RXR) family of steroid receptors and binding to specific DNA motifs termed PPAR-response elements (PPREs). PPAR-gamma is predominantly seen in adipose tissue where it plays a critical role in regulating adipocyte differentiation. Interestingly, both the orphan nuclear hormone receptor LXRα and thyroid receptor (TR) have been shown to act as antagonists of PPARα/RXRα binding to PPREs.
Description: Rabbit polyclonal to PPAR-gamma
Immunogen: KLH conjugated synthetic peptide derived from PPAR-gamma
Specificity: ·Reacts with Human, Mouse, Rabbit, Pig, Cow, Chicken and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 58 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
