Background: GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.
Description: Rabbit polyclonal to GAP-43
Immunogen: KLH conjugated synthetic peptide derived from GAP-43
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/200-500. Predicted Mol wt: 46 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
