Background: PACSINs are a family of cytoplasm-resisdent phosphoproteins that aid in vesicle formation and transport. It is presumed that all isoforms oligomerize and bind dynamin, synaptojanin 1 and N-WASP through their Src homology 3 domains. Furthermore, PACSINs colocalize with dynamin, but not with clathrin, indicating that the proteins may play a specific role with a defined population of dynamin at distinct cellular locations. PACSIN3 (Protein Kinase C and Casein Kinase Substrate in Neurons 3) contains a short proline-rich region and lacks asparagine-proline-phenylalanine motifs, which differentiates it from the rest of the PACSIN family. Sequence analysis of cDNAs encoding mouse and human PACSIN3 predict that the human protein consists of 424-amino acids and is 94% identical to the mouse protein. Studies of the mouse protein report predominant expression in mouse lung, skeletal muscle, and heart as well as in brain, kidney, and uterus.
Description: Rabbit polyclonal to PACSIN3
Immunogen: KLH conjugated synthetic peptide derived from PACSIN3
Specificity: ·Reacts with Human, Mouse and Rat.
.·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 48 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
