Background: PEBP-4 (phosphatidylethanolamine-binding protein 4), also known as Protein cousin-of-RKIP 1 (CORK1), is a 227 amino acid lysosomal protein that is a member of the RAF kinase inhibitory protein (RKIP) family. Unlike other RKIPs, PEBP-4 forms ternary complexes with Raf-1 and MEK and scaffolds this structure, thus resulting in the inhibition of the Raf-1/MEK/ERK signaling pathway. With preferential expression in muscle, PEBP-4 seems to control myocyte differentiation by modulation of MEK and ERK activity. Overexpression of PEBP-4 inhibits TNFα-induced activation of JNK and PE externalization, while reduced expression by siRNA knockdown results in an increase in TNFα-induced apoptosis. This suggests that PEBP-4 promotes cellular resistance to apoptosis and may be implicated in tumorigenesis.
Description: Rabbit polyclonal to PEBP4
Immunogen: KLH conjugated synthetic peptide derived from PEBP4
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 26 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
