Background: Folate is an essential vitamin that must be obtained from food intake through intestinal absorption in mammals. Folate and reduced folic acid derivatives bind to the folate receptor (FR) family, which mediates the endocytosis of 5-methyltetrahydofolate into the cell. MFT (Mitochondrial folate transporter/carrier), also known as Solute carrier family 25 member 32 (SLC25A32), is a 315 amino acid multi-pass membrane protein that regulates the influx of folate into the mitochondria. MFT also functions to complement glycine auxotrophy. Mutations in the gene encoding MFT may be involoved in certain cases of multiple acyl-CoA dehydrogenase deficiency (MADD), in which there is no mutation in EFTA or EFTB genes. MADD is a autosomal recessively inherited disorder in which fatty acid, amino acid and choline metabolism is disrupted, leading to a clinical manisfestation of hypoglycemia, hypotonia, hepatomegaly, metabolic acidosis and dysplastic kidneys.
Description: Rabbit polyclonal to MFT
Immunogen: KLH conjugated synthetic peptide derived from MFT
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 35 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
