Background: The activity of a diverse subset of enzymes relies on the essential nutrient copper. Copper uptake requires tight regulation to ensure that sufficient copper is present in the cell to drive vital cellular processes, while avoiding the accumulation of copper to toxic levels. In Saccharomyces cerevisiae, copper regulation involves several proteins. Fre1, a surface reductase, reduces and mobilizes copper outside the cell, while the Ctr1 and Ctr3 proteins function as copper transport proteins within the plasma membrane. Regulation of these proteins occurs at the transcriptional level. Under copper-deficient conditions, Mac1 binds to copper response elements (CuREs) within promoters, which contain the consensus sequence GCTC, to activate the transcription of Ctr1, Ctr3, and Fre1. Mac1 also mediates Ctr1 degradation. In human, Ctr1 also mediates the uptake of cisplatin, a chemotherapeutic drug, and may modulate the sensitivity and toxicity of this drug.
Description: Rabbit polyclonal to CTR1
Immunogen: KLH conjugated synthetic peptide derived from CTR1
Specificity: ·Reacts with Human, Mouse and Rat.
.·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 21 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
