Background: The biosynthesis of fatty acids can occur in the cytoplasm, via the type I fatty acid synthase complex, or in mitochondria, via the type II malonyl-CoA-dependent system. MCAT (mitochondrial malonyl CoA:ACP acyltransferase), also known as MT, MCT (mitochondrial malonyltransferase), fabD or FASN2C, is a member of the type II malonyltransferase family of proteins. Localizing to mitochondria, MCAT is encoded by a nuclear gene and, via an N-terminal localization signal, it is subsequently imported into mitochondria. MCAT functions in lipid metabolism and may be a component of a mitochondrial fatty acid synthase complex. More specifically, MCAT catalyzes the transfer of a malonyl group from malonyl-CoA to the mitochondrial acyl carrier protein (NDUFAB1), a subunit of respiratory complex 1. This reaction is essential in the initiation of the type II fatty acid biosynthesis system. Two isoforms of MCAT exist due to alternative splicing events.
Description: Rabbit polyclonal to MT
Immunogen: KLH conjugated synthetic peptide derived from MT
Specificity: ·Reacts with Human, Mouse and Rat.
.·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 43 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
