Background: The grainyhead subfamily, whose members include GRHL1, GRHL2 and GRHL3, consist of orthologs of the Drosophila grainyhead (GRH) protein. In Drosophila, GRH is involved in early dorsal/ventral patterning and tissue development. The grainyhead subfamily members are, therefore, believed to act as transcription factors during development. GRHL1, GRHL2 and GRHL3 are localized to the nucleus and exist as homodimers or as heterodimers with each other. GRHL1 (grainyhead-like 1 (Drosophila)), also known as MGR, LBP32, NH32 or TFCP2L2, is a 618 amino acid nuclear protein that is highly expressed in kidney, brain, tonsil, liver, placenta and pancreas. Like other members of the grainyhead family, GRHL1, which exists as three alternatively spliced isoforms, is thought to function as a transcription factor. GRHL1 is known to bind and transactivate the EN-1 promoter and may play a role in epidermal differentiation.
Description: Rabbit polyclonal to GRHL1
Immunogen: KLH conjugated synthetic peptide derived from GRHL1
Specificity: ·Reacts with Human, Mouse and Rat.
.·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 70 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
