Background: T lymphocytes express several low molecular mass transmembrane adaptor proteins that recruit SH2 domain-containing intracellular molecules to the cell membrane via tyrosine-based signaling pathways. One such protein, SIT (SHP2 interacting transmembrane adaptor protein) is a disulfide-linked homodimeric glycoprotein that is expressed in lymphocytes. SIT is reduced to half its molecular mass via endoglycosidase treatment. It contains five potential tyrosine phosphorylation sites, suggesting a role in TCR-mediated recruitment of SH2 domain-containing intracellular signaling molecules to the plasma membrane. SIT interacts with SHP2 and also with the adaptor protein Grb2. In addition, it is a substrate for the Src protein kinases Fyn, Lck and ZAP-70.
Description: Rabbit polyclonal to SIT
Immunogen: KLH conjugated synthetic peptide derived from SIT
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 21 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
